Intermediate

Part:BBa_K887022

Designed by: Weiting Chen   Group: iGEM12_NCTU_Formosa   (2012-09-10)

zif268+alss

Zif268 is a transcription factor with 88 amino acid residues,which form three zinc finger motifs. This transcription factor acts with other proteins to actively enhance or repress a gene by binding to the DNA recognition sequence 5’GCG-TGG-GCG 3’ at enhancer or repressor sites. AlsS is a coding region from Bacillus subtilis,and has mutated to have functional RE site.Alss encodes alpha-acetolactate synthase protein which is part of isobutanol biosynthesis pathway.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 608
    Illegal BsaI.rc site found at 1202


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Parameters
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